Journal of Cytology

ORIGINAL ARTICLE
Year
: 2011  |  Volume : 28  |  Issue : 4  |  Page : 159--164

Defining the validity of classical and non-classical cellular changes indicative of low-grade squamous intraepithelial lesion encompassing human papillomavirus infection in relation to human papillomavirus deoxyribonucleic acid testing


Veena Kashyap1, Suresh Hedau2, Suresh Bhambhani1 
1 Department of Cytology, Institute of Cytology and Preventive Oncology (DHR), Noida, UP, India
2 Department of Molecular Oncology, Institute of Cytology and Preventive Oncology (DHR), Noida, UP, India

Correspondence Address:
Veena Kashyap
Department of Cytology, Institute of Cytology and Preventive Oncology (DHR), Plot I-7, Sector-39, Noida, UP
India

Background: Human papillomavirus (HPV) infection as of now has been beyond doubt to be the causative agent for cervical carcinoma. Its morphological identification in Pap smear is important. Aim: To define the validity of classical and non-classical cellular changes indicative of low-grade squamous intraepithelial lesion (SIL) encompassing HPV infection in relation to positivity for «SQ»high risk«SQ» HPV16 as well as for «SQ»low risk«SQ» HPV6/11. Materials and Methods : A total of 3000 Papanicolaou smears were screened, of which 150 were reported as low grade-SIL encompassing HPV infection (LSIL-HPV). Subsequently cervical scrapes from these 150 subjects, along with equal number of normal women as controls, were collected and processed for HPV deoxy-ribonucleic acid testing by polymerase chain reaction (PCR). Results: On the basis of cytomorphological characteristics in Pap smears, HPV infection were categorized into the following two groups: Classical (koilocytic) changes (CC) encountered in 30 women and non-classical changes (NCC) encountered in 120 women. It was observed that 21 (70%) CC and 46 (38.3%) NCC of HPV infection were positive for HR-HPV16; however only 12 cases (10%) of NCC and two cases (6.6%) of CC were positive for LR-HPV 6/11. Majority (41.7%) of HPV positive cases were reported in the age group of 25 to 30 years and HPV positivity decreased with the increasing age. Conclusion: Classical cellular changes are not the only diagnostic features for HPV infection in Pap smear, non-classical diagnostic features also support the diagnosis of HPV infection and may be positive for HR-HPV16.


How to cite this article:
Kashyap V, Hedau S, Bhambhani S. Defining the validity of classical and non-classical cellular changes indicative of low-grade squamous intraepithelial lesion encompassing human papillomavirus infection in relation to human papillomavirus deoxyribonucleic acid testing.J Cytol 2011;28:159-164


How to cite this URL:
Kashyap V, Hedau S, Bhambhani S. Defining the validity of classical and non-classical cellular changes indicative of low-grade squamous intraepithelial lesion encompassing human papillomavirus infection in relation to human papillomavirus deoxyribonucleic acid testing. J Cytol [serial online] 2011 [cited 2021 Jan 23 ];28:159-164
Available from: https://www.jcytol.org/article.asp?issn=0970-9371;year=2011;volume=28;issue=4;spage=159;epage=164;aulast=Kashyap;type=0