Journal of Cytology
Home About us Ahead of print Instructions Submission Subscribe Advertise Contact e-Alerts Login 
Users Online:170
  Print this page  Email this page Small font sizeDefault font sizeIncrease font size
Year : 2020  |  Volume : 37  |  Issue : 1  |  Page : 26-29

“Agar Cell-Suspension”: A novel technique for processing clear specimens

Department of Cytopathology, Tata Memorial Hospital, Parel, Mumbai, Maharashtra, India

Correspondence Address:
Swati B Dighe
Department of Cytopathology, Tata Memorial Hospital, Parel, Mumbai - 400012, Maharashtra
Login to access the Email id

Source of Support: None, Conflict of Interest: None

DOI: 10.4103/JOC.JOC_199_18

Rights and Permissions

Context: Clear cytology specimens are processed by cytocentrifugation which is preferred over membrane filters (MF). Although both techniques are expensive, cytocentrifugation is less tedious and may cause cellular distortion. Aim: To standardize “Agar cell-suspension” (ACS) an innovative, simple, cost-effective technique to process clear specimens. Methods and Materials: About 93 clear specimens (65 urine, 15 effusion, 12 CSF, and 1 bronchial lavage) were processed by both cytocentrifugation and ACS. The sample was centrifuged in two tubes; one was used for ACS and other for cytocentrifugation. ACS smears were prepared by mixing one drop of 0.5% agar solution with the last drop of the centrifugate. Smears were fixed in methanol and stained by Papanicolaou staining method. ACS smears were compared with cytocentrifuged smears (CS) and evaluated for cellularity, cytomorphology preservation, staining quality, time, and cost. Results: As compared to CS smears, ACS smears showed better cellularity in 16.1%, comparable in 53.7%, and less in 30.1%. All ACS smears (100%) showed well-preserved cytomorphology as compared to 96.7% CS. Staining quality was optimal in 96.7% ACS smears against 91.3% CS. Both techniques took equal time. The additional cost of ACS was only 0.03 INR compared to 12.50 INR for CS. Conclusions: ACS is an innovative, simple, easy, and cost-effective technique for processing clear specimens. It gives equally good results comparable to cytocentrifugation in terms of cellularity and staining quality. ACS does not cause cell distortion or air-drying as seen in some CS. Thus, ACS is a superior alternative to cytocentrifugation.

Print this article     Email this article
 Next article
 Previous article
 Table of Contents

 Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
 Citation Manager
 Access Statistics
 Reader Comments
 Email Alert *
 Add to My List *
 * Requires registration (Free)

 Article Access Statistics
    PDF Downloaded136    
    Comments [Add]    

Recommend this journal