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  Indian J Med Microbiol
 

Figure 1: a) Pleural fluid smears showing numerous neutrophils, (Periodic acid Schiff stain x100). b) Smears showing positivity in neutrophils (Grocott methanamine silver stain x200) c) Smear showing positivity in neutrophil cytoplasm with lobulated unstained nuclei (Grocott methanamine silver stain x400) d) Pneumocystis jiroveci seen on Grocott stain. Careful observation will reveal lobated nuclei of neutrophils, whereas the organism will show typical “cup and saucer” appearance. (Grocott methanamine silver stain x 400)

Figure 1: a) Pleural fluid smears showing numerous neutrophils, (Periodic acid Schiff stain x100). b) Smears showing positivity in neutrophils (Grocott methanamine silver stain x200) c) Smear showing positivity in neutrophil cytoplasm with lobulated unstained nuclei (Grocott methanamine silver stain x400) d) <i>Pneumocystis jiroveci</i> seen on Grocott stain. Careful observation will reveal lobated nuclei of neutrophils, whereas the organism will show typical “cup and saucer” appearance. (Grocott methanamine silver stain x 400)