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  Indian J Med Microbiol
 

Figure 2: (a) Fluorescence in situ hybridization using dual-color probe for EWSR1 region (orange) and FLI1 region (green). A tumor cell of touch imprint smear showing one fused, one red and one green signal pattern. (b) Polyacrylamide gel electrophoresis of Reverse transcription polymerase chain reaction (RT-PCR) products. The present case showing a 166 bp band of type2 EWSR1 / FLI1 fusion (lane 1). (Lane M: DNA marker, lane 2: Negative control, lane 3: Fusion transcripts of a skeletal Ewing sarcoma, a 130 bp band of type 1 EWSR1/FLI1 fusion), (c) Sequence analysis of the RT-PCR product of the present case. EWSR1 exon 7 fused to FLI1 exon 5

Figure 2: (a) Fluorescence <i>in situ</i> hybridization using dual-color probe for <i>EWSR1</i> region (orange) and <i>FLI1</i> region (green). A tumor cell of touch imprint smear showing one fused, one red and one green signal pattern. (b) Polyacrylamide gel electrophoresis of Reverse transcription polymerase chain reaction (RT-PCR) products. The present case showing a 166 bp band of type2 <i>EWSR1</i> <i>/ FLI1</i> fusion (lane 1). (Lane M: DNA marker, lane 2: Negative control, lane 3: Fusion transcripts of a skeletal Ewing sarcoma, a 130 bp band of type 1 <i>EWSR1/FLI1</i> fusion), (c) Sequence analysis of the RT-PCR product of the present case. <i>EWSR1</i> exon 7 fused to <i>FLI1</i> exon 5