Journal of Cytology

ORIGINAL ARTICLE
Year
: 2020  |  Volume : 37  |  Issue : 3  |  Page : 122--125

Indigenous technique as an effective liquid-based cytology tool for multiple single-layered cell preparations


Shelly Sharma, Pranab Dey 
 Department of Cytology and Gynec Pathology, Post Graduate Institute of Medical Education and Research, Chandigarh, India

Correspondence Address:
Prof. Pranab Dey
Department of Cytology, Postgraduate Institute of Medical Education and Research (PGIMER), Chandigarh - 160012
India

Aims and Objectives: The aim of this work was to study the application of Modified Millipore technique (MMT) as a liquid-based tool to have multiple monolayer cellular smears for the routine cytology and Immunocytochemistry. Materials and Methods: In this study, we included 32 effusion fluid samples and 30 fine-needle aspiration cytology (FNAC) samples. From each of the samples, at first routine conventional smear was prepared. The residual samples were processed by MMT to make multiple smear preparation. Both the conventional and MMT of the monolayer cells were evaluated. The various cytomorphological features including the cellularity, background information, nuclear morphology, and cytoplasmic preservation were compared in these two techniques. Result: There were a total of 15 cases of fluid samples and 27 cases of FNAC. Statistical analysis of Mann–Whitney U test showed that the monolayer preparation by MMT and liquid-based cytology of fluid and FNAC smears are of same quality (P > 0.01). Conclusion: Monolayer preparation by MMT is cheap and effective. This technique can be used in routine laboratory for multiple monolayer cell preparations.


How to cite this article:
Sharma S, Dey P. Indigenous technique as an effective liquid-based cytology tool for multiple single-layered cell preparations.J Cytol 2020;37:122-125


How to cite this URL:
Sharma S, Dey P. Indigenous technique as an effective liquid-based cytology tool for multiple single-layered cell preparations. J Cytol [serial online] 2020 [cited 2020 Aug 12 ];37:122-125
Available from: http://www.jcytol.org/article.asp?issn=0970-9371;year=2020;volume=37;issue=3;spage=122;epage=125;aulast=Sharma;type=0