Journal of Cytology

ORIGINAL ARTICLE
Year
: 2019  |  Volume : 36  |  Issue : 1  |  Page : 8--12

Cytomorphological features of lung adenocarcinoma with anaplastic lymphoma kinase gene rearrangement


Saumya Shukla, Nuzhat Husain, Anurag Gupta, Nidhi Anand 
 Department of Pathology, Dr. Ram Manohar Lohia, Institute of Medical Sciences, Lucknow, Uttar Pradesh, India

Correspondence Address:
Dr. Nuzhat Husain
Department of Pathology, Dr. Ram Manohar Lohia Institute of Medical Sciences, Lucknow, Uttar Pradesh - 226 010
India

Background: The anaplastic lymphoma kinase (ALK) gene rearrangement is a predictive and prognostic marker in pulmonary adenocarcinoma. A series of clinical and pathological features have been documented in patients who harbor this translocation. Aims: The objectives of this study were to analyze the specific cytomorphological features of lung adenocarcinoma that harbored the ALK gene rearrangement and to compare the morphological features with the mutation-negative cases. Materials and Methods: The study sample of 40 cases included 15 ALK-positive cases and 25 ALK-negative cases. After the diagnosis was established, testing for ALK rearrangement was on the histopathology sample. The corresponding fine-needle aspiration cytology slides were retrieved from the records and cytomorphological features were analyzed. Results: A comparison of pattern between the ALK-positive and ALK-negative group revealed that the sheet-like pattern and singly scattered cells were more common in the ALK-positive group. Moderate-to-severe nuclear pleomorphism was identified in 80% of ALK-positive cases and 72% of ALK-negative cases. The presence of mucinous features and/or signet ring morphology was the most striking feature of ALK-positive cases with P value of 0.002. The presence of necrosis in the background was identified in 80% of ALK-positive cases and was statistically significant (P = 0.001). Conclusion: This is among the few studies from India, where a detailed analysis of the cytomorphological features of cases with ALK phenotype versus the mutation negative cases has been performed.


How to cite this article:
Shukla S, Husain N, Gupta A, Anand N. Cytomorphological features of lung adenocarcinoma with anaplastic lymphoma kinase gene rearrangement.J Cytol 2019;36:8-12


How to cite this URL:
Shukla S, Husain N, Gupta A, Anand N. Cytomorphological features of lung adenocarcinoma with anaplastic lymphoma kinase gene rearrangement. J Cytol [serial online] 2019 [cited 2019 Feb 22 ];36:8-12
Available from: http://www.jcytol.org/article.asp?issn=0970-9371;year=2019;volume=36;issue=1;spage=8;epage=12;aulast=Shukla;type=0