Journal of Cytology
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DNA extraction and polymerase chain reaction


 Department of Cytology and Gynaecological Pathology, Postgraduate Institute of Medical Education, Chandigarh, India

Correspondence Address:
Nalini Gupta,
Department of Cytology and Gynaecological Pathology, Postgraduate Institute of Medical Education and Research, Chandigarh - 160 012
India
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Source of Support: None, Conflict of Interest: None

DOI: 10.4103/JOC.JOC_110_18

DNA extraction and polymerase chain reaction (PCR) are the basic techniques employed in the molecular laboratory. This short overview covers various physical and chemical methods used for DNA extraction so as to obtain a good-quality DNA in sufficient quantity. DNA can be amplified with the help of PCR. The basic principle and different variants of PCR are discussed.



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