Journal of Cytology
Home About us Ahead of print Instructions Submission Subscribe Advertise Contact e-Alerts Login 
Users Online:256
  Print this page  Email this page Small font sizeDefault font sizeIncrease font size


 
 Table of Contents    
LETTERS TO EDITOR  
Year : 2019  |  Volume : 36  |  Issue : 2  |  Page : 133-134
Briskly motile Balantidium-like ciliate morphologically resembling Chilodonella spp. in urine sediment


1 Department of Pathology and Laboratory Medicine, AIIMS, Bhopal, Madhya Pradesh, India
2 Department of Pathology, GB Pant Institute of Postgraduate Medical Education and Research, New Delhi, India
3 Department of Microbiology, AIIMS, Bhopal, Madhya Pradesh, India
4 Department of Medicine, AIIMS, Bhopal, Madhya Pradesh, India

Click here for correspondence address and email

Date of Web Publication8-Mar-2019
 

How to cite this article:
Khurana U, Majumdar K, Karuna T, Joshi R, Kapoor N. Briskly motile Balantidium-like ciliate morphologically resembling Chilodonella spp. in urine sediment. J Cytol 2019;36:133-4

How to cite this URL:
Khurana U, Majumdar K, Karuna T, Joshi R, Kapoor N. Briskly motile Balantidium-like ciliate morphologically resembling Chilodonella spp. in urine sediment. J Cytol [serial online] 2019 [cited 2019 Jul 24];36:133-4. Available from: http://www.jcytol.org/text.asp?2019/36/2/133/253728




Sir,

A 69-year-old female came with vague complaints of weakness, insomnia, increased urinary frequency, burning micturition, and a history of recurrent urinary tract infections. She was on antihypertensives and antidepressants. A routine urine examination showed pale yellow urine with a pH of 7.0, specific gravity of 1.010, no glucosuria, proteinuria, or presence of nitrite. The centrifuged deposits revealed one to two epithelial cells, one to two pus cells, two to four red blood cells per high-power field (400×) and occasional calcium oxalate crystals. In addition, few briskly motile ciliates ranging in size from 30 to 40 μm in length and 20 μm in diameter were also appreciated. Finer details (the cilia, nucleus, and food vacuoles) of these parasites were better appreciable when the motility became sluggish. The body of the ciliate had right arched ciliary kinetics and left straighter ciliary kinetics, and motility was observed to be along one surface. The second urine examination after 3 days did not reveal any parasite. A third urine examination performed after another 2 days showed these ciliates were accompanied by flagellates. These flagellates were 12–15 μm in length, 8–10 μm in diameter, and had “falling leaf” motility. Stained centrifuged deposits showed finer morphological details of these ciliates and flagellates [Figure 1]. The sterility of the collection methods and containers was assured. No other patients evaluated during this time had any evidence of similar parasites. Based on their motility and cytomorphology, a diagnosis of co-infestation with Balantidium-like ciliate (resembling Chilodonella spp.) and Giardia spp. was considered. The indexed ciliate was smaller and had asymmetrical distribution of cilia in comparison to Balantidium. Stool examination carried on 2 consecutive days was negative for any parasite. The patient was treated with oral metranidazole 400 mg thrice daily for 7 days, and subsequent evaluation revealed normal urine sediments, free of any infestation.
Figure 1: (a) The ciliate showing ovoid macronucleus and a minor nucleus. Some cytoplasmic food vacuoles can be appreciated; note the scale indicating size (MGG; 400×). (b) They were asymmetrically oval shaped with convex aspect on one side and mild concavity on the other upper aspect forming a beak-like structure (PAP; 1000×). (c) The cilia appeared to be longer and better appreciable on one end (MGG; 1000×). (d) Two trophozoites of Giardia spp. (MGG; 400×)

Click here to view


Balantidium coli is the largest protozoon parasite and has been believed to be the only ciliate causing disease in man.[1] These organisms are known to cause dysentery with few reports documenting incidental infestation in urine and other body fluids.[1],[2] Elderly, debilitated, immunocompromised individuals and those with deficient personal hygiene are particularly susceptible. Close morphological differential diagnoses considered in this particular case included Balantidium-like ciliates, namely, Colpoda spp.,[3] Chilodonella spp.,[4] and Buxtonella spp.[5] If the size of the organism is around 100 μm or more, Balantidium spp. is more likely; however, the differential appears to be broader with lesser size of around 40–60 μm. In latter situations, it seems rational to designate as Balantidium-like ciliates. It is almost impossible to categorize the specific genus of these ciliates without gene amplification and sequencing, the facility of which is available in a few isolated centers.[5] Chilodonellids are small ciliated protozoans found worldwide as free-living species in water and they are a common fish parasite infesting in their gills and skin.[4] The parasite in this index case had resemblance to Chilodonella and human infestation has not been reported. The patient did not give history of any diarrheal episodes, but the water being used for bathing was from the well, which may be a source of colonization by the organism. The presence of Giardia spp. in urine may be due to fecal contamination.

To conclude, human infestation by Balantidium-like ciliates is rare and thorough evaluation of morphology and motility, supplemented by molecular methods, may elucidate the potential pathogenic role of rare ciliates other than Balantidium in humans.

Financial support and sponsorship

Nil.

Conflicts of interest

There are no conflicts of interest.



 
   References Top

1.
Schuster FL, Ramirez-Avila L. Current world status of Balantidium coli. Clin Microbiol Rev 2008; 21:626-38.  Back to cited text no. 1
    
2.
Bandyopadhyay A, Majumder K, Goswami BK. Balantidium coli in urine sediment: Report of a rare case presenting with hematuria. J Parasit Dis 2013;37:283-5.  Back to cited text no. 2
    
3.
Costache C, Bursasiu S, Filipas C, Colosi I. A case of ciliate protozoa Colpoda spp (Ciliata: Colpodidae) detected in human urine. Iranian J Parasitol 2011;6:99-104.  Back to cited text no. 3
    
4.
Mitra AK, Haldar DP. First record of Chilodonella hexasticha (Kiernik, 1909) Kahl, 1931 (Ciliophora: Chilodonellidae) infesting a freshwater fish Nandus nandus (Hamilton) from Gangetic West Bengal, India. Anim Biol 2004;54:111-8.  Back to cited text no. 4
    
5.
Pomajbikova K, Obornik M, Horak A, Petrzelkova KJ, Grim JN, Levecke B. Novel insights into the diversity of Balantidium and Balantidium like cyst forming ciliates. PLoS Negl Trop Dis 2013;7:e2140.  Back to cited text no. 5
    

Top
Correspondence Address:
Dr. Kaushik Majumdar
Associate Professor, Department of Pathology, GB Pant Institute of Postgraduate Medical Education and Research, New Delhi
India
Login to access the Email id

Source of Support: None, Conflict of Interest: None


DOI: 10.4103/JOC.JOC_21_18

Rights and Permissions


    Figures

  [Figure 1]



 

Top
 
 
  Search
 
  
    Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
    Email Alert *
    Add to My List *
* Registration required (free)  


    References
    Article Figures

 Article Access Statistics
    Viewed233    
    Printed5    
    Emailed0    
    PDF Downloaded27    
    Comments [Add]    

Recommend this journal