Journal of Cytology
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 Table of Contents    
CASE REPORT  
Year : 2017  |  Volume : 34  |  Issue : 1  |  Page : 61-63
Endometriosis mimicking glandular atypia in a cervical cytology


1 Department of Pathology and Laboratories, Hospital Universitario Fundación Santa Fe de Bogotá; School of Medicine, Universidad de los Andes, Colombia
2 Department of Pathology and Laboratories, Hospital Universitario Fundación Santa Fe de Bogotá, Colombia

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Date of Web Publication6-Jan-2017
 

   Abstract 

Endometriosis involving the uterine cervix is a rare condition that can lead to diagnostic errors in the interpretation of Pap smear. We report the case of a 41-year-old patient in whom the initial Pap smear revealed three-dimensional clusters of glandular cells with elongated nuclei, occasional mitosis, and atypia, which was interpreted as atypical glandular cells, not otherwise specified (NOS). The patient was taken to colposcopy and endocervical biopsy. Colposcopy was normal and the biopsy presented glands with elongated nuclei and surrounded by endometrial stroma admixed with normal endocervical glands. Immunohistochemical studies were reactive for CD10 in the stromal cells and vimentin in endometrioid glands. The findings were consistent with cervical endometriosis. Endometriosis in the cervix is an uncommon pathology that mimics malignancy and may be interpreted as atypical or glandular neoplasia in the cytology.

Keywords: Atypical glandular cells of undetermined significance (AGUS); cervical cytology; endometriosis

How to cite this article:
Rodriguez-Urreg PA, Dulcey-Hormiga IC, Barrera-Herrera LE, Suarez-Zamora DA, Palau-Lazaro MA, Buritica-Cifuentes C. Endometriosis mimicking glandular atypia in a cervical cytology. J Cytol 2017;34:61-3

How to cite this URL:
Rodriguez-Urreg PA, Dulcey-Hormiga IC, Barrera-Herrera LE, Suarez-Zamora DA, Palau-Lazaro MA, Buritica-Cifuentes C. Endometriosis mimicking glandular atypia in a cervical cytology. J Cytol [serial online] 2017 [cited 2017 Mar 26];34:61-3. Available from: http://www.jcytol.org/text.asp?2017/34/1/61/197624



   Introduction Top


Endometriosis is defined as the presence of endometrial glands and stroma in ectopic localizations outer than the uterine cavity. The most frequent site of implantation is the ovaries and pelvic cavity, followed by the bowel, bladder, umbilical region, skin, and perianal region. [1] Endometriosis of the uterine cervix is uncommon and may have different morphologies, such as superficial, deep, and polypoid. [2],[3] Superficial endometriosis can be detected in cervicovaginal cytology and lead to errors in interpretation as atypical glandular cells. [4],[5] We report the case of a patient with superficial endometriosis, detected incidentally on a routine cervical cytology interpreted as atypical glandular cells, and correlation with histopathology.


   Case History Top


A 41-year-old patient, G3P0A0, with previous history of two obstetric curettages due to retained abortions and previous negative Pap smears, attended to her routine annual Pap smear screening. The cervical cytology revealed tridimensional hyperchromatic glandular groups with nuclear overlapping [Figure 1]a, round nuclei in the center, and palisading nuclei on the periphery of the cluster with occasional mitosis [Figure 1]b and c; no feathering was seen. The atypical glandular cells were considered to be endocervical in origin, and were interpreted as atypical glandular cells, not otherwise specified (NOS).
Figure 1: (a) Tridimensional hyperchromatic groups (H&E, x200), (b) Glandular group shows round overlapping nuclei with occasional mitosis and palisading nuclei at the periphery (H&E, x400). (c) Atypical glandular cells with hyperchromatic palisading nuclei (H&E, x400). (d) Cervical biopsy with central foci of ectopic endometrial glands surrounded by endometrial stroma and lined in the periphery by unremarkable endocervical glands (H&E, x100). Immunohistochemistry, shows (e) reactive endometrial glands and negative endocervical glands with vimentin (Vimentin, x100) and (f) reactive endometrial stroma with CD10 (CD10, x100)

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Patient was subjected to colposcopy that was negative, following which she was taken for endocervical biopsy. The sample was fixed in buffered formalin 10%, and slides were stained with hematoxylin and eosin. Tissue sections revealed foci of ectopic endometrioid glands surrounded by endometrial stroma [Figure 1]d admixed with benign endocervical glands. Immunohistochemistry was reactive for vimentin on endometrioid glands [Figure 1]e and for CD10 in endometrial stoma [Figure 1]f, but was negative in endocervical glands. Findings were consistent with cervical endometriosis.


   Discussion Top


Since 2001 when the Bethesda system was incorporated into the classification of glandular abnormalities, the need to classify endocervical or endometrial samples was investigated by multiple studies that reported the difficulty in the interpretation of these lesions and the high interobserver variability. [6],[7]

Incidence of cervical endometriosis has been reported to be between 0.7 and 2.4% of colposcopies. [8] The morphology may range from shallow-to-deep and polypoid cells that can be detected in Pap smear and can lead to an interpretation of atypical glandular cells, mimicking endocervical neoplasia, as the morphology of endometrial cells may present a wide morphologic spectrum, making the histopathological examination definitive in defining the final diagnosis.

Similar to previous reports, the most common finding, as in the presented case, was the three-dimensional clusters of glandular cells and cytologic atypia with slight increase in nuclear size and occasional mitosis. [1] The differential diagnosis of atypical glandular cells apart from endometriosis includes benign conditions such as tubal metaplasia and lower uterine segment sampling and malignant conditions as adenocarcinoma in situ of the endocervix, and endometrial adenocarcinoma. [1],[9]

To the best of our knowledge, there are only 48 cases reported of endometriosis initially observed in the cytology with definitive diagnosis in the biopsy. The history of previous gynecological procedures together with atypical groups lining the normal endocervix in the absence of apoptosis, mitosis, and feathering of the cytoplasm are useful to suspect that the findings are mimicking malignancy.

Financial support and sponsorship

Nil.

Conflicts of interest

There are no conflicts of interest.

 
   References Top

1.
Barkan GA, Naylor B, Gattuso P, Kullu S, Galan K, Wojcik EM. Morphologic features of endometriosis in various types of cytologic specimens. Diagn Cytopathol 2013;41:936-42.  Back to cited text no. 1
    
2.
Baker PM, Clement PB, Bell DA, Young RH. Superficial endometriosis of the uterine cervix: A report of 20 cases of a process that may be confused with endocervical glandular dysplasia or adenocarcinoma in situ. Int J Gynecol Pathol 1999;18:198-205.  Back to cited text no. 2
    
3.
Felix A, Nogales FF, Arias-Stella J. Polypoid endometriosis of the uterine cervix with Arias-Stella reaction in a patient taking phytoestrogens. Int J Gynecol Pathol 2010;29:185-8.  Back to cited text no. 3
    
4.
Lundeen SJ, Horwitz CA, Larson CJ, Stanley MW. Abnormal cervicovaginal smears due to endometriosis: A continuing problem. Diagn Cytopathol 2002;26:35-40.  Back to cited text no. 4
    
5.
Symonds DA, Reed TP, Didolkar SM, Graham RR. AGUS in cervical endometriosis. J Reprod Med 1997;42:39-43.  Back to cited text no. 5
    
6.
Simsir A, Hwang S, Cangiarella J, Elgert P, Levine P, Sheffield MV, et al. Glandular cell atypia on Papanicolaou smears: Interobserver variability in the diagnosis and prediction of cell of origin. Cancer 2003;99:323-30.  Back to cited text no. 6
    
7.
Lee KR, Darragh TM, Joste NE, Krane JF, Sherman ME, Hurley LB, et al. Atypical glandular cells of undetermined significance (AGUS): Interobserver reproducibility in cervical smears and corresponding thin-layer preparations. Am J Clin Pathol 2002;117:96-102.  Back to cited text no. 7
    
8.
Veiga-Ferreira MM, Leiman G, Dunbar F, Margolius KA. Cervical endometriosis: Facilitated diagnosis by fine needle aspiration cytologic testing. Am J Obstet Gynecol 1987;157:849-56.  Back to cited text no. 8
    
9.
Phadnis SV, Doshi JS, Ogunnaike O, Coady A, Padwick M, Sanusi FA. Cervical endometriosis: A diagnostic and management dilemma. Arch Gynecol Obstet 2005;272:289-93.  Back to cited text no. 9
    

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Correspondence Address:
Paula A Rodriguez-Urreg
Department of Pathology and Laboratories, Carrera 7 No. 117-15 (Second Floor), Fundación Santa Fe de Bogotá, Bogotá
Colombia
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0970-9371.197624

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