Journal of Cytology
Home About us Ahead of print Instructions Submission Subscribe Advertise Contact e-Alerts Login 
Users Online:178
  Print this page  Email this page Small font sizeDefault font sizeIncrease font size


 
 Table of Contents    
CASE REPORT  
Year : 2017  |  Volume : 34  |  Issue : 1  |  Page : 45-48
Penicillium marneffei infection in a HIV-Positive patient: A comparison of bronchial washing cytology and biopsy


Department of Pathology, University of Ulsan College of Medicine, Asan Medical Center, Seoul, Korea

Click here for correspondence address and email

Date of Web Publication6-Jan-2017
 

   Abstract 

Penicilliosis is a disseminated and progressive infection that is mainly found in immunocompromised individuals, especially those with human immunodeficiency virus (HIV) infection. Because of the high mortality of patients with disseminated Penicillium marneffei infection, rapid diagnosis and early treatment are required. Diagnosis is traditionally made by biopsy and/or culture of blood or any involved organ. Cytology offers several advantages over biopsy, including more rapid diagnosis and greater resolution of cytomorphologic details of organisms, allowing rapid initiation of treatment. Here, we describe a case of penicilliosis in an HIV-positive patient with emphasis on the morphological characteristics of the organism in cytologic specimens, as well as a comparison of bronchial washing and biopsy findings.

Keywords: Bronchial washing cytology; HIV; penicilliosis

How to cite this article:
Hee CH, Pil CY, Cho KJ. Penicillium marneffei infection in a HIV-Positive patient: A comparison of bronchial washing cytology and biopsy. J Cytol 2017;34:45-8

How to cite this URL:
Hee CH, Pil CY, Cho KJ. Penicillium marneffei infection in a HIV-Positive patient: A comparison of bronchial washing cytology and biopsy. J Cytol [serial online] 2017 [cited 2017 Mar 26];34:45-8. Available from: http://www.jcytol.org/text.asp?2017/34/1/45/197618



   Introduction Top


Penicilliosis, a disseminated and progressive infection caused by Penicillium marneffei, is endemic in Southeast Asia, including Thailand, Southern China, Hong Kong, Vietnam, Indonesia, and Laos. The organism was first reported in 1956 and the first human infection was described in 1973 in an American who lived in Southeast Asia and was diagnosed with Hodgkin lymphoma. In 1988, the first human immunodeficiency virus (HIV)-associated case was reported, and, since then, penicilliosis has been regarded as an hAIDS-indicating disease." [1] Because of nonspecific clinical manifestations, penicilliosis in HIV-infected patients can be easily misdiagnosed as other infections, such as tuberculosis, histoplasmosis, and cryptococcosis, and malignancy. Penicilliosis is associated with a high mortality rate, which has been reported to be 13.5% (7/52) in HIV-infected patients and 60% (6/10) in nonortality rate. [2],[3]

Here, we report a case of penicilliosis in an HIV-positive patient who was diagnosed after bronchial washing and biopsy, with emphasis on the morphological characteristics of the organism in a cytologic specimen.


   Case Report Top


A 42-year-old male patient visited the emergency room of our hospital in November 2013 with fever, sore throat, and diarrhea. He was a known seropositive patient for HIV and syphilis and a frequent traveler to Guangxi, China, and Hong Kong. Laboratory testing on admission showed a white blood cell count of 9600/μo (8764/μh, neutrophils, 470/μh lymphocytes), 7.0 g/dL hemoglobin, and 86 K/μh platelets. The peripheral CD4+ T lymphocyte count was 25 cells/μp. Computed tomography (CT) scans revealed bilateral septal thickening and micronodules of both the lungs, severe hepatosplenomegaly, and multiple enlarged lymph nodes in the chest and abdominal cavity, with tuberculosis or malignancy, especially lymphoma, suspected. Bronchoscopy revealed multiple micronodules in the wall of the right bronchi. Bronchoscopic biopsy and washing cytology with concurrent supraclavicular lymph node biopsy were performed.

The bronchial washing cytology was diffuse and cellular smear contained mixed inflammatory infiltrate, including numerous histiocytes, lymphocytes, some eosinophils, and neutrophils. Most histiocytes were packed with many yeast-like organisms measuring 3mm in diameter that stained dark brown on methenamine silver (GMS) stain [Figure 1]. Focusing up and down through histiocytes, the organisms appeared to have a central dot-like structure. Bronchoscopic biopsy revealed anergic tissue reactions with histiocytic infiltration. In Hematoxylin and eosin (H and E)-stained sections, the organisms were seen as nonbudding round-to-oval spherules within the histiocytes in the submucosa of bronchi [Figure 2]. These organisms were also observed extracellularly but not in bronchial epithelial cells. In the lymph node, there was lymphoid depletion with diffuse proliferation of histiocytes engorged with many organisms.
Figure 1: Bronchial washing cytology shows bronchial epithelial cells and mixed acute and chronic inflammatory infiltrate, including numerous histiocytes. Most histiocytes were packed with many yeast-like organisms (Pap, ×400) with inset showing oval to oblong in shape with central transverse septum (GMS, ×1000)

Click here to view
Figure 2: Bronchoscopic biopsy shows diffuse histiocytic proliferation with organisms in submucosa. (H&E, ×400)

Click here to view


On the basis of cytologic and histologic findings, the initial cytopathologic interpretation suggested histoplasmosis. Two days after admission, the mycelial form presented in an interim report on a fungal culture obtained from the blood. Given its dimorphic feature and the patient's history of travel to endemic areas, penicilliosis was suspected and a polymerase chain reaction assay was positive for P. marneffei. We reviewed the bronchial washing and biopsy findings. In the cytologic specimen, while intracellular organisms appeared to be uniform yeasts of 3 μs in size, extracellular fungi were elongated and up to 8 μi in length (sausage form). The most characteristic feature was a central transverse septum that was transparent with Papanicolaou stain but highlighted by GMS stain [Figure 1]. The size, shape, and distribution of the organisms were similar in both biopsy and cytologic specimens, except that central dots and septa were more readily identified on cytologic smears.

After amphotericin B administration, the patient exhibited clinical and radiologic improvement. Amphotericin B was changed to oral itraconazole 400 mg/day after 2 weeks. Toxoplasma IgG, influenza, and pneumococcal-conjugated (PCV 13) vaccines were also prescribed to prevent opportunistic infections. The patient continues to visit the outpatient clinic without any specific problems.


   Discussion Top


Penicilliosis, an emerging opportunistic infection in the Southeast Asian region, especially among patients with HIV infection, is the third most common "ost commonis, an emergi," following tuberculosis and cryptococcosis in frequency. [1],[4] Diagnosis is difficult due to nonspecific clinical manifestations that match those of other generalized infections, such as tuberculosis, histoplasmosis, and cryptococcosis, or malignancy. Identification of a characteristic thermal dimorphism on fungal culture is diagnostic for penicilliosis, however, it is a time-consuming method for proper management of critically ill patients.

Diagnosis can be quicker using biopsy or cytology specimens when pathologists are aware of the epidemiology and morphology of P. marneffei infection. The histopathology of penicilliosis can lead to three distinct tissue reactions depending on the host immunity. A granulomatous or suppurative reaction occurs in immunocompetent patients, whereas an anergic and necrotizing reaction is often observed in patients with compromised immunity. In infected organs, P. marneffei can be observed both extracellularly and intracellularly, mainly within histiocytes. Our case showed diffuse submucosal histiocytic infiltrate with numerous intracellular granular organisms. In contrast to the report of Chongrak et al., no P. marneffei were detected within bronchial epithelial cells in either surgical biopsy or cytologic specimens. [5]

Cytologic specimens are less invasively and more easily obtained than tissue biopsy. Although the primary goal of cytology is to screen for malignancy, inflammation, and reparative changes, other abnormalities may also be detected during specimen examination. The morphologic diagnosis of fungal infection is another important function of cytology. [6] A key observation in our current case is that morphologic evidence of P. marneffei was more apparent in cytologic specimens than that in biopsy. The main advantage of cytologic preparations in P. marneffei infection is that the Papanicolaou stain (a routine stain in cytology) specifically highlights the central transverse septum, unlike H and E stain (a routine stain in histology). This accounts for greater resolution of the cytomorphologic details of routine cytology for identifying P. marneffei because cytology lacks formalin-fixation artifacts and has less crush artifacts than biopsy. Another advantage of cytology over histology is that noncohesive histiocytes engorged with P. marneffei may be better visualized. Thus, cytology may be superior to biopsy for the detection of P. marneffei.

In addition to the morphologic comparison, another important consideration is diagnostic yield. Because the present case was diagnosed with both modalities, the comparative diagnostic yield of biopsy versus cytology cannot be addressed. P. marneffei infection has been diagnosed by fine-needle aspiration of lymph nodes, sputum cytology, touch preparation from skin scrape, and bronchoalveolar lavage. [7],[8],[9] A review of the literature reveals variable findings of lung cytology. The differences may be due to the method adopted, which ranges from bronchial washing, sputum, percutaneous lung aspirate, and bronchoalveolar lavage. [6],[7],[8],[9] Although the diagnostic yield of bronchial washing cytology for P. marneffei has been reported as zero, bronchial washing from our case showed diagnostic features on review. [6] Thus, we believe that bronchial washing cytology is comparable to that of other lung specimens.

Other fungi with cytomorphologic findings similar to P. marneffei include Histoplasma capsulatum and Pneumocystis jirovecii. Histoplasmosis is most commonly confused with penicilliosis, as in our case. H. capsulatum yeast divides by budding, not fission, and shows a more uniformly round shape, not an oval or sausage form. An epidemiologic link to an area of endemicity of the two fungi can also help in the diagnosis. P. jirovecii is mainly recognized in an extracellular location and in crescent forms; the organisms lack septation. [7],[10]


   Conclusion Top


P. marneffei causes a disseminated infection and can be the initial manifestation in HIV-infected patients. Because the number of HIV-positive patients is increasing around the world, we need to be aware of P. marneffei as a potential pathogen in immunocompromised patients. Mortality in patients with disseminated P. marneffei infection is high, regardless of HIV infection status, hence, rapid diagnosis and early treatment are required. In cytologic specimens, the diagnosis is made by linking the detection of oval or sausage-shaped yeast-like organisms with characteristic central transverse septum to a clinical history of visits to endemic areas.

Financial support and sponsorship

Nil.

Conflicts of interest

There are no conflicts of interest.

 
   References Top

1.
Vanittanakom N, Cooper CR Jr, Fisher MC, Sirisanthana T. Penicillium marneffei infection and recent advances in the epidemiology and molecular biology aspects. Clin Microbiol Rev 2006;19:95-110.  Back to cited text no. 1
    
2.
Wu TC, Chan JW, Ng CK, Tsang DN, Lee MP, Li PC. Clinical presentations and outcomes of Penicillium marneffei infections: A series from 1994 to 2004. Hong Kong Med J 2008;14:103-9.  Back to cited text no. 2
    
3.
Wong SS, Wong KH, Hui WT, Lee SS, Lo JY, Cao L, et al. Differences in clinical and laboratory diagnostic characteristics of penicilliosis marneffei in human immunodeficiency virus (HIV)- and non-HIV-infected patients. J Clin Microbiol 2001;39:4535-40.  Back to cited text no. 3
    
4.
Supparatpinyo K, Khamwan C, Baosoung V, Nelson KE, Sirisanthana T. Disseminated Penicillium marneffei infection in Southeast Asia. Lancet 1994;344:110-3.  Back to cited text no. 4
    
5.
Spira C, Mitchai J, Thongsri W, Spira B. Diagnostic cytology and morphometry of Penicillium marneffei in the sputum of a hypogammaglobulinemia with hyper-IgM patient. J Med Assoc Thai 2010;93(Suppl 3):S69-72.  Back to cited text no. 5
    
6.
Jan IS, Chung PF, Wang JY, Weng MH, Hung CC, Lee LN. Cytological diagnosis of Penicillium marneffei infection. J Formos Med Assoc 2008;107:443-7.  Back to cited text no. 6
    
7.
Chaiwun B, Khunamornpong S, Sirivanichai C, Rangdaeng S, Supparatpinyo K, Settakorn J, et al. Lymphadenopathy due to Penicillium marneffei infection: Diagnosis by fine needle aspiration cytology. Mod Pathol 2002;15:939-43.  Back to cited text no. 7
    
8.
Supparatpinyo K, Chiewchanvit S, Hirunsri P, Uthammachai C, Nelson K, Sirisanthana T. Penicillium marneffei infection in patients infected with human immunodeficiency virus. Clin Infect Dis 1992;14:871-4.  Back to cited text no. 8
    
9.
Chan JK, Tsang DN, Wong DK. Penicillium marneffei in bronchoalveolar lavage fluid. Acta Cytol 1989;33:523-6.  Back to cited text no. 9
    
10.
Tsui WM, Ma KF, Tsang DN. Disseminated Penicillium marneffei infection in HIV-infected subject. Histopathology 1992;20:287-93.  Back to cited text no. 10
    

Top
Correspondence Address:
Kyung Ja Cho
Department of Pathology, University of Ulsan College of Medicine, Asan Medical Center, Seoul - 138-736
Korea
Login to access the Email id

Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0970-9371.197618

Rights and Permissions


    Figures

  [Figure 1], [Figure 2]



 

Top
 
 
  Search
 
  
    Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
    Email Alert *
    Add to My List *
* Registration required (free)  


    Abstract
   Introduction
   Case Report
   Discussion
   Conclusion
    References
    Article Figures

 Article Access Statistics
    Viewed500    
    Printed5    
    Emailed0    
    PDF Downloaded18    
    Comments [Add]    

Recommend this journal