Journal of Cytology
Home About us Ahead of print Instructions Submission Subscribe Advertise Contact e-Alerts Login 
Users Online:205
  Print this page  Email this page Small font sizeDefault font sizeIncrease font size


 
 Table of Contents    
CASE REPORT  
Year : 2012  |  Volume : 29  |  Issue : 2  |  Page : 139-141
Malignant solitary fibrous tumor in the extremity: Cytopathologic findings


Department of Pathology, Salah Azaďez Institute, Tunis, Tunisia

Click here for correspondence address and email

Date of Web Publication12-Jun-2012
 

   Abstract 

Malignant solitary fibrous tumor (SFT) is an extremely rare neoplasm. There are only rare published accounts of the cytopathologic features of this tumor. We report a case of a 59-year-old woman presented with a 10-year history of a right thigh mass. A preoperative fine needle aspiration (FNA) was performed. Smears were hypercellular, with cohesive and crowded tissue fragments, haphazard cell arrangements and many single cells. The tumor cells were polymorphous, plump spindled or round with often indented or bare nuclei. A differential diagnosis of low grade sarcoma was favored. The diagnosis of malignant SFT is extremely difficult on FNA and must be included in the differential diagnosis of spindle cell neoplasms.

Keywords: Cytopathology; malignant; solitary fibrous tumor; soft tissue; extremity

How to cite this article:
Khanchel F, Driss M, Mrad K, Romdhane KB. Malignant solitary fibrous tumor in the extremity: Cytopathologic findings. J Cytol 2012;29:139-41

How to cite this URL:
Khanchel F, Driss M, Mrad K, Romdhane KB. Malignant solitary fibrous tumor in the extremity: Cytopathologic findings. J Cytol [serial online] 2012 [cited 2020 Sep 22];29:139-41. Available from: http://www.jcytol.org/text.asp?2012/29/2/139/97160



   Introduction Top


Malignant solitary fibrous tumors (SFT) are extremely rare mesenchymal tumors. [1] Originally thought to occur exclusively in intrathoracic sites, they are now known to be quite ubiquitous. [2] There is very little literature regarding the cytologic findings in this tumor. In the current study we describe the cytologic features of malignant SFT arising in the right thigh.


   Case Report Top


A 59-year-old woman presented with a 10-year history of a right thigh mass. An ultrasound examination showed a solid mass of 10 × 10 cm with heterogeneous echotexture and rich vascularity. Magnetic resonance imaging scan (MRI) showed inhomogeneous low intensity signal on T1-weighted image and intermediate intensity signal on T2-weighted image of 6 cm in the anterior thigh. A preoperative FNA aspiration was performed using 22- and 25-gauge needles and air-dried and alcohol-fixed smears were made. The air dried-smears were stained by May-Grünwald-Giemsa (MGG) stain and alcohol-fixed smears were stained with Papanicolaou stain.

Cytologic findings

Smears were hypercellular, with cohesive and crowded tissue fragments, haphazard cell arrangements, and many single cells in a background of dense collagen bands and capillary blood vessels [Figure 1]a. The tumor cells were polymorphous, plump spindled or round with often indented or bare nuclei [Figure 1]b. A diagnosis of low-grade sarcoma was favored. The patient underwent a complete resection of the tumor.
Figure 1: FNA of malignant SFT (a) A large hypercellular, haphazard cell arrangements and many single cells. The tumor cells are polymorphous, plump spindled or round with often indented or bare nuclei (MGG, ×400) (b) Single oval to spindle cells within a background of dense collagenous bands (Pap stain, ×400)

Click here to view


Pathologic findings

At gross examination, the tumor was 10×5, 5×3 cm in size. It was firm and well circumscribed. The cut surface was reddish and whorled [Figure 2]a.
Figure 2: (a) Gross appearance of malignant SFT (b) Histologic section: patternless growth and hemangiopericytoma-like vessels (H and E, ×200) juxtaposed with (c) areas displaying malignant features such as hypercellularity, pleomorphism and mitosis (H and E, ×400) (d) Immunohistochemical studies: The tumor cells are diffusely positive for CD34 (IHC, ×250)

Click here to view


Pathological examination showed a highly cellular neoplasm. The tumor showed a patternless architecture with branching hemangiopericytoma-like vessels [Figure 2]b. The tumor cells were relatively monomorphic ovoid or sometimes spindle cells showing a moderate nuclear pleomorphism. Mitotic rate was 8 mitoses per 10 high-power fields [Figure 2]c.

Immunohistochemical studies revealed a heterogeneous cytoplasmic positivity for CD34 [Figure 2]d and a strong cytoplasmic positivity for vimentin, bcl-2 and CD99.

On the basis of the cytological, histological and immunohistochemical findings this case was classified as malignant SFT.


   Discussion Top


The increasingly wide spread use of FNA for soft tissue lesions necessitate the recognition of even rare neoplasms. [3] SFTs are uncommon mesenchymal tumors with usually benign behavior. Nevertheless 10% to 15% of SFTs will recur and/or metastasize. [4] The soft tissues of the extremities are among the rarest sites of occurrence of this entity. In this location SFT is likely to have a malignant potential. [5]

Cytologic features of SFT are relatively non specific, comprising of hypocellular clusters of cohesive, bland spindled cells with inconspicuous nucleoli in a collagenic and hemorrhagic background with staghorn vessels. These features are better seen in cell block preparations. [3] In the few series describing cytologic features of malignant SFT the clue of malignancy are hypercellularity, pleomorphism and epithelioid or round cell features, as well as necrosis and mitoses. [3] However, hypercellularity and even necrosis have also been described in rare cases of benign SFT. [4] Some authors suggested that a predominance of single cells as features would favor malignancy. [4] In our case cytologic features of malignancy were hypercellularity, pleomorphism and many single cells. There were neither mitoses nor necrosis.

Grossly, malignant SFTs are large with an average of 13.4 cm, (the tumor in our case sized 10 cm), consistent with the notion that malignant SFTs are generally larger than benign SFTs. [1] Hemorrhage and/or necrosis, common gross features of malignant neoplasms, were not seen in our case. [3]

Microscopically, the typical benign SFTs, display zones of both hypercellular and hypocellular collagenized stroma in a so called patternless architecture. [1] It is characterized by a branching hemangiopericytoma-like (staghorn) vasculature. [1] Criteria of malignancy are not standardized, but malignant SFT is described by the World Health Organization classification of soft tissue tumors as having features including hypercellularity, at least focal moderate to marked cellular atypia, tumor necrosis, ≥ 4 mitoses/10 high-power fields, and infiltrative margins. [2]

The differential diagnosis of malignant SFT in the extremity, include a spectrum of benign or low-grade malignant spindle cell lesions such as hemangiopericytoma, benign and malignant neurogenic tumors, fibrosarcoma, synovial sarcoma (SS) and dermatofibrosarcoma protuberans (DFSP). The CD34 may prove a useful positive marker in cytology. However, it is not necessarily specific. [6]

Probably the most difficult diagnosis to distinguish from malignant SFT is benign SFT. Flint and Weiss [7] reported CD34 positivity in 77% of benign solitary fibrous tumors and 100% of malignant SFT. However, Ali et al[6] described a loss of CD34 expression in malignant SFTs. Our case showed an heterogeneous expression of this antigen. Bcl-2, which has been reported to be more consistently positive than CD34 in malignant SFT, was positive in our case. [7]

SFTs are usually negative for S-100 protein, actins, desmin and cytokeratins. However, focal immunoreactivity for cytokeratins, actins, S-100 protein, epithelial membrane antigen may be seen. [1]

Hemangiopericytoma shows a monotonous pattern with a staghorn configuration. A feature of "patternless pattern" is a characteristic of solitary fibrous tumors and cannot be detected in hemangiopericytoma. It is diffusely positive for CD 34. Peripheral nerve sheath tumors are characterized by a pain during aspiration biopsy procedure. [8] They show focally positive immunoreactivity to CD34 antibody in dendritic cells. [9] Benign peripheral nerve sheath tumors diffusely express S100 protein. This expression may be lost in 30-50% of malignant peripheral nerve sheath tumors. [10] The appearance of a monotonous population with spindled and/or round cells may be reminiscent of SS. If present, glandular structures of a biphasic SS exclude the possibility of malignant SFT. Immunohistochemically, both malignant SFT and SS share bcl-2 positivity, but any significant positivity for CD34 effectively excludes the diagnosis of SS. [3] Fibrosarcoma present the pattern of a uniform population of spindle cells, both dispersed and arranged in clusters of fascicular structures. [8] They do not show positive immunoreactivity to CD34 antibody. [8] Smears from a malignant SFT show cytologic and immunohistochemical patterns similar to dermatofibrosarcoma protuberans (DFSP). Compared with SFT, DFSP is less deeply located and involves the dermis.

In conclusion, malignant SFT in the extremity is rare. It is crucial to correlate the cytomorphologic features of FNA with the clinical presentation and the radiologic findings. It shares some features with benign SFT, and malignancy is usually difficult to assess in the absence of mitoses and necrosis. Because of its non specific appearance, it is most often not possible to accurately diagnose malignant SFT on FNA material.

 
   References Top

1.Guillou L, Fletcher JA, Fletcher CD, Mandahl N. Extrapleural solitary fibrous tumor and hemangiopericytoma. In: Fletcher CD, Unni KK, Mertens F, editors. Pathology and genetics of tumours of soft tissues and bone. Lyon: IARC Press; 2002. p. 86-8.  Back to cited text no. 1
    
2.Rosai J. Soft tissues. Rosai and Ackerman's surgical pathology. London: Mosby; 2004. p. 2248-9.  Back to cited text no. 2
    
3.Bishop JA, Rekhtman N, Chun J, Wakely PE Jr, Ali SZ. Malignant solitary fibrous tumor: cytopathologic findings and differential diagnosis. Cancer Cytopathol 2010;118:83-9.  Back to cited text no. 3
[PUBMED]  [FULLTEXT]  
4.Vallat-Decouvelaere AV, Dry SM, Fletcher CD. Atypical and malignant solitary fibrous tumors in extrathoracic locations: evidence of their comparability to intra-thoracic tumors. Am J Surg Pathol 1998;22:1501-11.  Back to cited text no. 4
[PUBMED]  [FULLTEXT]  
5.Wan S, Ning L, Hong R, Wu W, Fan S, Tsuchiya H, et al. Clinicopathological features of solitary fibrous tumours in the extremities: four case reports and a literature review. J Int Med Res 2010;38:694-704.  Back to cited text no. 5
[PUBMED]  [FULLTEXT]  
6.Fine SW, McCarthy DM, Chan TY, Epstein JI, Argani P. Malignant solitary fibrous tumor of the kidney: report of a case and comprehensive review of the literature. Arch Pathol Lab Med 2006;130:857-61.  Back to cited text no. 6
[PUBMED]  [FULLTEXT]  
7.Flint A, Weiss SW. CD-34 and keratin expression distinguishes solitary fibrous tumor (fibrous mesothelioma) of pleura from desmoplastic mesothelioma. Hum Pathol 1995;4:428-31.  Back to cited text no. 7
    
8.Weiss SW, Nickoloff BJ. CD-34 is expressed by a distinctive cell population in peripheral nerve, nerve sheath tumors, and related lesions. Am J Surg Pathol 1993;17:1039-45.  Back to cited text no. 8
[PUBMED]    
9.Baliga M, Flowers R, Heard K, Siddiqi A, Akhtar I. Solitary fibrous tumor of the lung: a case report with a study of the aspiration biopsy, histopathology, immunohistochemistry, and autopsy findings. Diagn Cytopathol 2007;35:239-44.  Back to cited text no. 9
[PUBMED]  [FULLTEXT]  
10.Louis DN, Ohgaki H, Wiestler OD, Cavenee WK, editors: WHO classification of tumours of the central nervous system. Lyon: IARC; 2007.  Back to cited text no. 10
    

Top
Correspondence Address:
Fatma Khanchel
Department of Pathology, Salah Azaďez Institute, 1006 Bab Saadoun, Tunis
Tunisia
Login to access the Email id

Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0970-9371.97160

Rights and Permissions


    Figures

  [Figure 1], [Figure 2]

This article has been cited by
1 Frozen Cytology of Meningeal Malignant Solitary Fibrous Tumor/Hemangiopericytoma
Myunghee Kang,Na Rae Kim,Dong Hae Chung,Gie-Taek Yie
Journal of Pathology and Translational Medicine. 2019; 53(3): 192
[Pubmed] | [DOI]



 

Top
 
 
  Search
 
  
    Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
    Email Alert *
    Add to My List *
* Registration required (free)  


    Abstract
   Introduction
   Case Report
   Discussion
    References
    Article Figures

 Article Access Statistics
    Viewed2717    
    Printed98    
    Emailed2    
    PDF Downloaded83    
    Comments [Add]    
    Cited by others 1    

Recommend this journal