Journal of Cytology
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ORIGINAL ARTICLE Table of Contents   
Year : 2007  |  Volume : 24  |  Issue : 3  |  Page : 130-133
Cytological diagnosis of infections in renal allograft recipients


Kamineni Hospitals, L.B. Nagar, Hyderabad, India

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   Abstract 

Infection is the most common cause of mortality and morbidity within the first year of transplantation. Renal allograft recipients are susceptible to various opportunistic infections because of prolonged immunosuppression. The study was carried out to evaluate the role of cytology for rapid diagnosis of infections in transplant patients. Fine needle aspiration cytology (FNAC) of superficial lesions, guided aspirations from deep seated lesions, bronchoalveolar lavage, endoscopic brushings and urine cytology was the material studied. 292 renal transplants were done during a period of nine years. 46 cases of infections were diagnosed, comprising of bacterial (26), viral (2), fungal (13) and mixed bacterial and fungal (5). Cytological examination offers a rapid and specific diagnosis of infections in these immunocompromised patients. The incidence, severity of infection and mortality in post transplant patients will decrease with early diagnosis and high index of clinical suspicion.

Keywords: Post transplant infections, cytological diagnosis

How to cite this article:
Radha S, Tameem A, Fernandez D K. Cytological diagnosis of infections in renal allograft recipients. J Cytol 2007;24:130-3

How to cite this URL:
Radha S, Tameem A, Fernandez D K. Cytological diagnosis of infections in renal allograft recipients. J Cytol [serial online] 2007 [cited 2019 Sep 16];24:130-3. Available from: http://www.jcytol.org/text.asp?2007/24/3/130/41902



   Introduction Top


Infections occurring after transplantation account for half of the deaths in post transplant patients. During the first post transplantation year, 40-50% of recipients experience atleast one infection. The incidence of infection is directly related to the net immunosuppressive effect achieved. Infections most commonly occur in mucocutaneous areas (41%), urinary tract (17.2%) and respiratory tract (13.9%). The common infective agents are bacterial, viral, fungal and protozoal. In the first month infections are directly related to surgery, the next six months are associated with high levels of immunosuppression and carry a risk for opportunistic infections. Early diagnosis and prolonged treatment are required for eradication of these infectious agents. Cytological material can provide a rapid, sensitive and specific diagnosis of infections. It also provides material for ancillary studies. Fine needle aspiration cytology (FNAC) has been reported to be a highly sensitive and specific diagnostic tool in the management of post-transplant patients.[1] When correctly used, bronchoalveolar lavage too is a safe and useful procedure with a high diagnostic yield in immunocompromised host.[2]




   Materials and Methods Top


This study was done in department of pathology over a period of nine years (1997-2005). During this period 292 renal transplants were done from live related donors and cadavers. Immunosuppression was induced with three doses of methyl prednisolone followed by tapering doses over three months. They were maintained on cyclosporine and azathioprine. High risk transplants recipients were induced with basiliximab. All the transplant recipients received antiviral prophylaxis with acyclovir from day one of transplant for three months and antifungal prophylaxis with flucanazole for two months. FNAC of superficial lesions, guided aspirations from deep seated lesions, bronchoalveolar lavage (BAL), endoscopic brushings and urine cytology was the material studied. Smears were stained with Haematoxylin and Eosin (H&E). Stains for acid fast bacilli (AFB) and fungus were done for all the cases.


   Observations Top


We diagnosed forty six cases of infections on cytology. Male to female ratio was 2.8:1 and age of the patients ranged from 21 to 69 years. Pulmonary infections were the commonest infections in our series. Commonest presentation was fever with respiratory symptoms. Skin lesions varied from single to multiple cutaneous abscesses. Basic cytological inflammatory responses like acute inflammation, chronic inflammation, granulomatous inflammation and necrosis were observed. Tissue response in lesions diagnosed and confirmed with Ziehl Neelsen stain as tuberculous lesions was both acute inflammatory and granulomatous.

Candidiasis was diagnosed based on the presence of small elliptical yeast and pseudohyphae. The lesions diagnosed as zygomycetes had broad sparsely septate hyphae that branched haphazardly. Aspergillus lesions revealed thin regular hyphae which were septate and branched at acute angles. The morphology of all the fungal lesions was confirmed on silver stains.

The tissue response in the single case of cytomegalovirus (CMV) was mononuclear infiltration; infected cells were enlarged with a large basophilic intranuclear inclusion. Decoy cells in BK virus nephropathy revealed an enlarged nucleus with a smudged basophilic appearance. The lesions of Pneumocystis revealed foamy exudates on H&E and the helmet shaped cysts were seen on silver stains. Nocardial lesions revealed thin branching irregular bacilli on silver stains and these were weakly AFB positive. Clinical and cytological details are given in [Table 1].


   Discussion Top


Major complication of renal transplant recipients is infection. During the first post transplant year 40%50% of transplant recipients experience at least one infection. Multiple factors including tropical climate, diabetes, chronic hepatitis and late presentation contribute to poor outcome. The time table for occurrence of infections is shown in [Table 2]. [3]

In our study bacterial infections occurred within the first two weeks of transplantation. Nocardial lesions occurred two months to one year and viral infections occurred two years after transplantation. Fungal infections occurred from six month to three years. Of solid organ transplants, renal transplant is associated with lowest rates of infection. This is because renal transplant is an elective or semi elective procedure. [4] In our study 46 patients developed infections out of 292 transplants. Out of the five nonspecific bacterial infections, two patients had perirenal abscesses and two had lower respiratory tract infections and one patient had a cutaneous abscess.

Sixteen patients had mycobacterial infections. Fifteen lung lesions and one lymphnodal lesion were diagnosed as mycobacteriosis [Figure 1]. Five patients had other associated infections. AFB was demonstrated in all the cases and culture was done in two cases. Both the cultures yielded Mycobacterium tuberculosis. The incidence of tuberculosis in post transplant cases is 10-15% as against 1-2% in general population. [5] The mortality in post transplant patients from tuberculosis is 10.5-23%. In our study all the ten patients with isolated tuberculous lesions recovered with initiation of anti tuberculous therapy. Five patients with combined infections expired. One patient was lost to follow up. Mortality correlates with the duration of symptoms prior to antituberculous treatment, and therefore early recognition and treatment is essential as the course of the disease can be rapidly fatal in tuberculosis. [6]

Twelve cases had fungal infections and in five cases, it was associated with other infections. Ten lesions were from lung and two were from cutaneous abscesses. The species identified were aspergillosis, zygomycosis and candidiasis. One patient was diagnosed on BAL to have pneumocystis. The incidence of fungal infections in live renal transplant recipients is 6.1%. [7] Candidiasis and aspergillosis were common infections as was reported by other workers. [8] The risk factors for fungal infections are multifactorial. In our patients risk factors included immunosuppressive therapy and diabetes mellitus. The mortality due to fungal infections is 65%. [9] The mortality in our series was 50%. The outcome of fungal infections may improve with high index of clinical suspicion, early diagnosis and appropriate treatment.

Five patients had nocardial abscesses, four were from the lung and one was a cutaneous abscess [Figure 2]. Among renal transplants recipients nocardiosis is a marker of high susceptibility to tuberculosis and other infections. The association with tuberculosis is high in patients if the infections develop within two years of transplant. [10] In two of our cases there was associated tuberculosis.

Two patients were diagnosed to have viral infections, one was cytomegalovirus which was diagnosed on endoscopic brush cytology and the other was polyoma virus. The latter was suspected on urine cytology and was confirmed on a graft biopsy. The prevalence of CMV in transplant patients is 21.8%. [11] Infection in our patient had a relationship with an acute rejection episode. Sakhuja et al [11] had documented CMV in 12 of the 69 autopsies between 1992-1997. In our study only one case of CMV was diagnosed on cytology and the rest were diagnosed on biopsies. Urine cytology of one patient with deteriorating graft function revealed decoy cells and his graft biopsy revealed features of polyoma virus nephropathy. BK virus associated interstitial nephritis is a significant cause of allograft dysfunction in 2-5% of patients. The disorder has poor prognosis and approximately 50% of allografts failing over subsequent months. [12]

To conclude, infections remain the major hazard of transplantation in India. The incidence, severity of infection and mortality in post transplant patients will decrease with early diagnosis and high index of clinical suspicion. Different cytological techniques play an important role in achieving this goal.

 
   References Top

1.Siddiqui MT, Reddy VB, Castelli MJ, Gattuso P. Role of fine needle aspiration in clinical management of transplant patients. Diagn Cytopathol 1997; 17 : 429-35.  Back to cited text no. 1  [PUBMED]  [FULLTEXT]
2.Abramson MJ, Stone CA, Holmes PW, Tai EH. The role of bronchoalveolar lavage in the diagnosis of suspected opportunistic pneumonia. Aust N Z J Med 1987; 17 : 407-12.  Back to cited text no. 2  [PUBMED]  
3.Fishman JA, Rubin RH. Infection in organ transplant recipients. N Engl J Med 1998; 338 : 1741-51.  Back to cited text no. 3  [PUBMED]  [FULLTEXT]
4.Kuback BM. Infectious complications of kidney transplantation and their management. In: Danavitch GM, editor. Hand book of kidney transplantion. Philadelphia: Lippincott Williams & Wilkins; 2002. p. 221-62.  Back to cited text no. 4    
5.John GT. Infections after renal transplantation in India. Transplantation Reviews 1999; 13 : 183-91.  Back to cited text no. 5    
6.David TY Lam, HL Tang, KL Tong. Tuberculosis in post-transplant patients. Hong Kong Med J 1992; 44 : 169-75.  Back to cited text no. 6    
7.Chugh KS, Sakhuja V, Jain S, et al. High mortality in systemic fungal infections following renal transplantation in third world countries. Nephrol Dial Transplant 1993; 8 : 168-72.  Back to cited text no. 7  [PUBMED]  [FULLTEXT]
8.Bren A, Koselj M, Kandus A, et al. Severe fungal infections in kidney graft recipients. Transplant Proc 2002; 34 : 2999-3000.  Back to cited text no. 8  [PUBMED]  [FULLTEXT]
9.Jha V, Chugh KS. Post transplant infections in tropical countries. Artif Organs 2002; 26 : 770-7.  Back to cited text no. 9  [PUBMED]  [FULLTEXT]
10.John GT, Shankar V, Abraham MA, Mathew M, Thomas PP, Jacob CK. Nocardiosis in tropical renal transplant recipients. Clin Transplant 2002; 16 : 285-9.  Back to cited text no. 10    
11.Sakhuja V, Jha V, Joshi K, Nada R. Increasing incidence of cytomegalovirus disease in renal transplant recipients on cyclosporine immunosuppression. Transplant Proc 2001; 33 : 3631.  Back to cited text no. 11    
12.Preiksaitis JK, Cockfield SM, Trpkov K. Polyomavirus infection and disease in renal transplant recipients. Current Opinion in Organ Transplantation 2001; 6 : 151-8.  Back to cited text no. 12    

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S Radha
Plot No.:20, Road No.:1, Alakapuri, Hyderabad - 500035, Andhra Pradesh
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0970-9371.41902

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