Journal of Cytology
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ORIGINAL ARTICLE
Year : 2000  |  Volume : 17  |  Issue : 1  |  Page : 15-22

A comparison of methods to improve quality of smears in bloody cell samples of serous fluids


1 Scientific Officer ‘SF’, In-charge, Cytology Laboratory, Tata Memorial Hospital, Dr. Ernest Borges Road, Parel, Mumbai - 400 012, India
2 Scientific Assistant ‘B’, Cytology Laboratory, Tata Memorial Hospital, Dr. Ernest Borges Road, Parel, Mumbai - 400 012, India

Correspondence Address:
Sylla G Malvi
In-charge, Cytology Laboratory, Tata Memorial Hospital, Parel, Mumbai-12
India
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Source of Support: None, Conflict of Interest: None


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The presence of RBC partially or completely obscuring the morphologic details of cells in serous fluids, makes it difficult for the cytolog ist to give an accurate diagnosis. Many methods have been suggested for treatment and fixation of haemorrhagic fluids. In view of our restricted resources, we have tried out three methods selected on the basis of simplicity of processing, low cost and easy availability of reagents. The three methods selected were: haemolysis by a) glacial acetic acid, b) Carnoy's fixative and c) normal saline rehydration technique. A comparison was made between the three methods for lysis of RBC on thirty randomly selected grossly bloody serous fluid samples. Various parameters such as an improvement in diagnostic accuracy, ty of nuclear features were compared. The saline rehydration technique was judged to be superior to the other two. RBC whe persistence of RBC in the background and the effect of the lysing agent on claritere completely lysed and there was no distortion of nuclear features of epithelial cells in the large majority of saline treated cases.


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